Issue 12, 2023
From the journal:

RSC Chemical Biology

Quantitative mass spectrometric analysis of hepatocellular carcinoma biomarker alpha-fetoprotein†

Chen-Chun Chen,‡ab   Han-Wen Huang,‡b   Bo-Rui Chenb  and  Chi-Huey Wong ORCID logo *bc  

* Corresponding authors

a Department of Chemistry, National Taiwan University, Taipei, Taiwan

b Genomic Research Center, Academia Sinica, Taipei, Taiwan

c Department of Chemistry, The Scripps Research Institute, 10550 N. Torrey Pines Rd., La Jolla, CA 92037, USA
E-mail: wong@scripps.edu

Abstract

Serum alpha-fetoprotein (AFP) has been used as a marker for the diagnosis of hepatocellular carcinoma (HCC) and its core fucosylation is associated with the early stage of HCC. However, current methods for the detection of AFP with core fucose are not highly accurate for early diagnosis. In this study, we established an enzyme-assisted mass spectrometric method for the quantitative analysis of AFP/core fucose with high specificity and sensitivity. We employed endoglycosidase treatment of AFP to improve the biomarker analysis. The accuracy and precision are within the US FDA-suggested value, and a good linearity (r2 = 0.9930) and a detection limit of 15.6 ng mL−1 can be achieved.

Graphical abstract: Quantitative mass spectrometric analysis of hepatocellular carcinoma biomarker alpha-fetoprotein

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Article information

DOI
https://doi.org/10.1039/D3CB00069A
Article type
Paper
Submitted
17 May 2023
Accepted
22 Aug 2023
First published
09 Oct 2023
This article is Open Access
Creative Commons BY-NC license

RSC Chem. Biol., 2023,4, 1073-1081

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Quantitative mass spectrometric analysis of hepatocellular carcinoma biomarker alpha-fetoprotein

C. Chen, H. Huang, B. Chen and C. Wong, RSC Chem. Biol., 2023, 4, 1073 DOI: 10.1039/D3CB00069A

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